Composite

Part:BBa_K4773014:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


J23100 Promoter+RBS+hps+RBS+phi+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


Design Notes

Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation.Different restriction sites were added between each part to facilitate the connection of each part or further manipulation. RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.


Source

hps&phi: From the genomic sequence of Bacillus subtilis.

References